SOLiD Samples Submission
Please fill the SOLiD 5500 Samples Submission Form and send it before submitting any samples.
If you need help with filling the form please read the instructions how to fill in the form.Samples Requirements
Library type | Optimal amount | Volume
|
Fragment | 1-5 µg | upto 100 µl
|
ChIP | 10 ng - 1 µg | 10-50 µl
|
Mate-Pair | 5-20 µg | upto 100 µl
|
Targeted Enrichment | 3-6 µg | upto 100 µl
|
Whole transcriptome | ||
- total RNA | 5-10 µg | upto 10 µl
|
- rRNA-depleated RNA or - poly(A)RNA | 200-500 ng | upto 10 µl
|
Small RNA | 5-10 µg of total RNA | upto 50 µl
|
SAGE | 2-5 µg of total RNA | 10-50 µl |
- DNA quantitation must be done by fluorometry (e.g. PicoGreen-based).
- Purified samples with an A260/A280 of ~1.8 can be provided in water or Qiagen EB buffer.
- Please provide an image of a gel for each sample with clearly indicated sample identity and ladder bands size or Bioanalyzer electropherogram.
IMPORTANT: The reference sequence must be e-mailed to FGCZ. For data QC expected primer sequences must be also sent to FGCZ.
Submitted samples will be stored for four weeks after data delivery. During this time the material unused for analysis can be taken back. After this period, any biological material will be discarded without further notification.